Grid preparation and data collection for cryo-EM
نویسندگان
چکیده
منابع مشابه
Persistent topology for cryo-EM data analysis.
In this work, we introduce persistent homology for the analysis of cryo-electron microscopy (cryo-EM) density maps. We identify the topological fingerprint or topological signature of noise, which is widespread in cryo-EM data. For low signal-to-noise ratio (SNR) volumetric data, intrinsic topological features of biomolecular structures are indistinguishable from noise. To remove noise, we empl...
متن کاملGraDeR: Membrane Protein Complex Preparation for Single-Particle Cryo-EM.
We developed a method, named GraDeR, which substantially improves the preparation of membrane protein complexes for structure determination by single-particle cryo-electron microscopy (cryo-EM). In GraDeR, glycerol gradient centrifugation is used for the mild removal of free detergent monomers and micelles from lauryl maltose-neopentyl glycol detergent stabilized membrane complexes, resulting i...
متن کاملCryo-EM of viruses and vaccine design.
Knowledge of the atomic resolution structures of viruses can be a powerful tool for vaccine discovery and design. X-ray crystallography has long served as an invaluable method for virus structure determination at high resolution, but over the past decade, cryoelectron microscopy (cryo-EM) has begun to emerge as a complementary method that can also provide this kind of information. Crystallograp...
متن کاملResilient Data Collection in Smart Grid
Sensors and measurement devices are widely deployed in Smart Grid (SG) to monitor the health of the system. However, these devices are subject to damage and attack so that they cannot deliver sensing data to the control center. In treebased data collection schemes, a relay failure can further lead to unresponsiveness of all the devices in its sub-tree. In this paper, we study the resiliency iss...
متن کاملAn automatic method for predicting transmembrane protein structures using cryo-EM and evolutionary data.
The transmembrane (TM) domains of many integral membrane proteins are composed of alpha-helix bundles. Structure determination at high resolution (<4 A) of TM domains is still exceedingly difficult experimentally. Hence, some TM-protein structures have only been solved at intermediate (5-10 A) or low (>10 A) resolutions using, for example, cryo-electron microscopy (cryo-EM). These structures re...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations and Advances
سال: 2019
ISSN: 2053-2733
DOI: 10.1107/s0108767319097344